This condensed course is aimed at everybody, who wants to understand the basic principles of optics and fluorescence in microscopy. The course if organised by the Core Facility for Integrated Microscopy at the Department of Biological Sciences at the University of Copenhagen, Denmark. For more information please visit the specific page of the event https://cfim.ku.dk/events/light-microscopy-two-day-crash-course-january-2022/
Theoretical lectures are followed by practicals. The course will cover:
Basic Principles of microscopy
- Optics of the microscope
- Diffraction, Resolution and Contrast
Fluorescence and Confocal Microscopy
- Fluorescence microscopy (fluorophores, illumination…)
- Basic Confocal microscopy (lasers, multi-dimensional image acquisition, live imaging …)
Details
Minimum / Maximum number of participants:
12 / 18
Register here.
The course will be confirmed two-weeks before it should take place, and free cancellation is only allowed 3 weeks before it takes place.
Prices: Academic 2500 DKK / Commercial 5000 DKK
ECTs Credits: Ph.D. students will be entitled to apply for ECTs credits via this form.
Provisional programme:
| Day 1 | |
| 09:00 | Light, Resolution and Magnification |
| 09:45 | The Microscope, Conjugate Planes and Contrast techniques |
| 10:45 | Coffee break |
| 11:00 | Abbe’s diffraction experiments (Video – Peter Evennett) |
| 11:20 | Practical 1 (3 rotations – 30min each)Aperture & Conjugated planes on Optical bench (LP)Resolution (THB)Köhler illumination and conjugated planes (CP) |
| 12:50 | Lunch break |
| 13:30 | Introduction to fluorescence |
| 14:15 | Coffee break |
| 14:30 | Light sources, detectors and principles of confocal microscopy |
| 15:00 | Practical 2, 3 groups in parallel – 1h Intro to confocal microscopes, setting Köhler and DIC on LSM |
| Day 2 | |
| 09:00 | Laser scanning confocal microscopy Colocalization analysis – important considerations |
| 09:45 | Practical considerations before image acquisition Live Imaging strategies |
| 11:00 | Coffee break |
| 11:15 | Practical 3, rotation 1 – 1h30 Testing resolution and optimizing dynamic rangeAcquiring confocal data – multi channel designSpinning Disk and TIRF |
| 12:45 | Lunch |
| 13:30 | Practical 3, rotation 2 – 1h30 |
| On the run | Coffee |
| 15:00 | Practical 3, rotation 3 – 1h30 |
