Light microscopy two-day crash course – March 31 – April 1, 2022

This condensed course is aimed at everybody, who wants to understand the basic principles of optics and fluorescence in microscopy. The course if organised by the Core Facility for Integrated Microscopy at the Department of Biological Sciences at the University of Copenhagen, Denmark. For more information please visit the specific page of the event

Theoretical lectures are followed by practicals. The course will cover:

Basic Principles of microscopy

  • Optics of the microscope
  • Diffraction, Resolution and Contrast

Fluorescence and Confocal Microscopy

  • Fluorescence microscopy (fluorophores, illumination…)
  • Basic Confocal microscopy (lasers, multi-dimensional image acquisition, live imaging …)


Minimum / Maximum number of participants:
12 / 18

Register here.

The course will be confirmed two-weeks before it should take place, and free cancellation is only allowed 3 weeks before it takes place.

Prices: Academic 2500 DKK  / Commercial  5000 DKK

ECTs Credits: Ph.D. students will be entitled to apply for ECTs credits via this form.

Provisional programme:

Day 1
09:00Light, Resolution and Magnification
09:45The Microscope, Conjugate Planes and Contrast techniques
10:45Coffee break
11:00Abbe’s diffraction experiments (Video – Peter Evennett)
11:20Practical 1 (3 rotations – 30min each)Aperture & Conjugated planes on Optical bench (LP)Resolution (THB)Köhler illumination and conjugated planes (CP)
12:50Lunch break
13:30Introduction to fluorescence
14:15Coffee break
14:30Light sources, detectors and principles of confocal microscopy
15:00Practical 2,  3 groups in parallel – 1h
Intro to confocal microscopes, setting Köhler and DIC on LSM
Day 2
09:00Laser scanning confocal microscopy
Colocalization analysis – important considerations
09:45Practical considerations before image acquisition
Live Imaging strategies
11:00Coffee break
11:15Practical 3, rotation 1 – 1h30
Testing resolution and optimizing dynamic rangeAcquiring confocal data – multi channel designSpinning Disk and TIRF
13:30Practical 3, rotation 2 – 1h30
On the runCoffee
15:00Practical 3, rotation 3 – 1h30

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