New Imaging and Fluctuation Methods: Super-Resolution, Light-Sheet, STED-FCS and FRET-FCS

Light-Sheet Imaging

• Long time-lapse imaging of live modelorganisms with low phototoxicity
• Combined light-sheet and super-resolution

Super-Resolution Imaging in Living Cells – MoNaLISA

• Smart probes
• 50 nm resolution at 1-2 Hz
• 100 x 100 um field of view time lapse imaging

STED-FCS

• Fluctuation spectroscopy with 30 nm resolution
• Sub-diffraction dynamics in living cells

FRET-FCS

• Easy detection of protein-protein interactions in living cells
• Highly sensitive detection of rare oligomers in living cells

Lecturers

Andreas Bodén
Erdinc Sezgin
Ilaria Testa
Stefan Wennmalm, stewen@kth.se

Web-based PhD-level course, 3 hp credits, by the ALM facility at Scilifelab.

Registration

Please register by sending an e-mail with your contact details to: stewen@kth.se.

Registration deadline: May 20, 2022