PhD course in New Super-Resolution, Light-Sheet, and FCS-Methods at SciLifeLab Stockholm , Sweden – October 14th-25th, 2024

The Advanced Light Microscopy Facility, ALM, at Scilifelab gives a two-week PhD-level course on four new imaging and fluorescence spectroscopy techniques:

Depletion-based Super-Resolution Imaging

  • STED-imaging, 2D and 3D, 30-50 nm resolution
  • In Living Cells – MoNaLISA, 1-2 Hz, 100 x 100 mm field of view

MINFLUX

  • Imaging with 3 nm resolution
  • Tracking single molecules at 10 kHz

Light-Sheet Imaging

  • Long time-lapse imaging of live model organisms with low phototoxicity
  • Ultrafast volumetric imaging of cells with lattice light-sheet microscopy

FCS-Methods

  • Measurements of concentrations, diffusion coefficients, molecular dynamics and interactions, in solution or living cells
  • Cross-correlation, FRET-, STED-, and line-scan-FCS are covered

Lecturers:

Steven Edwards

Erdinc Sezgin

Ana Agostinho

Francesca Pennacchietti

Hans Blom

Stefan Wennmalm

Dates: Monday October 14th – Friday October 25th 2024

Credits: 3 hp for PhD students

Location: Gamma 3, Scilifelab, Solna

Registration: stewen@kth.se latest September 30th